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KMID : 0376219850220030411
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Chonnam Medical Journal
1985 Volume.22 No. 3 p.411 ~ p.429
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An Ultrastructural Study on Granulomatous Tissue Evoked by Silicone Elastomer
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Abstract
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Epithelioid and multinucleated giant cells, characteristic for granulomatous reaction, are derived from monocytes of the bone marrow origin, and granulomatous lesions are observed around foreign bodies or indigestible material as well as in cell-mediated delayed hypersensitivity reaction. Besides, the multinucleated giant cells in foreign body granuloma often contain phagocytes particulates. The epitheliod and multinucleated giant cells, however, have limited phagocytic activity. To get an insight into these seemingly paradoxical phenomena, silicone elastomer, a biologically inert material, was inserted into the subcutaneous layer of the rats and the tissue reacted to silicone was examined light and electron microscopically, 1, 2, 3 and 4 weeks after the insertion. The results were summarized as follows.
1. Silicone was encapsulated by granulomatous tissue which was again surrounded by granulation or fibrous tissue. Epithelioid and foreign body giant cells were observed mainly in the inner layer of the granulomatous tissue adjacent to silicone and Longhand giant cells in the outer layer of the granulomatous tissue and granulation or fibrous tissue. Cellular composition of the capsule was more or less similar during the examination period, though differences in quantity between granulomatous reaction and fibrosis were noted.
2. The Epithelioid cells, roughly polygonal in shape, had many filopodia. The abundant cytoplasm contained well developed rough endoplasmic reticulum, the cistern of which were dilated and contained finely granular substance of low electron density, many polyribosomes and mitochondria. Lysosomes were noted occasionally but silicone phagosomes were not.
3. The nuclei of the foreign body giant cells were scattered in the cytoplasm and the organelles occasionally were compacted around them. Rough endoplasmic reticulum was most prominent, having vesicular structure, and polyribosomes and mitochondria were plenty. Longhand giant cells showed internal organization of the organelles; polyribosomes were located in the periphery of the cytoplasm where the nuclei were arranged in 1 or 2 rows, and rough endoplasmic reticulum and mitochondria in the central area. Multinucleate cells of both types in the granulomatous tissue contained only a few lysosomes and scarce silicone phagosomes.
These results indicated that the Epithelioid cells and multinucleate giant cells might be the cells of serectory type rather than those having phagocytic activity, and that development of these cells might have no direct relationship with phagocyte features. It was also suggested that the phagocytosed particulates in the multinucleate giant cells resulted mainly from fusion of the macrophages having phagocytic features.
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KEYWORD
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